Jensen Ackles is CHRIS REDFIELD
Natassia Malthe is JILL VALENTINE
Justin Hartley is LEON S. KENNEDY
Scout Taylor-Compton is CLAIRE REDFIELD
Christian Bale is ALBERT WESKER
Mei Melançon is ADA WONG
Ayumi Hamasaki is REBECCA CHAMBERS
Jason Statham is BARRY BURTON
Rodrigo Santoro is CARLOS OLIVEIRA
Crispin Glover is DR. WILLIAM BIRKIN
Supporting Actors:
Abigail Breslin is Sherri Birkin
Charlize Theron is Annette Birkin
Mekhi Pfeifer is Marvin Branagh
Morgan Freeman IS Chief Brian Irons/NARRATOR