Crispin Glover as the Joker
Joaquin Phoenix as Harvey Dent/Two-Face
Johnny Depp as Eddy Nygma/Riddler
Tim Curry as Oswald Cobblepot/Penguin
Jeremy Renner as John Crane/Scarecrow
Charlize Theron as Selina Kyle/Catwoman
Jena Malone as Harleen Quinzel/Harley Quinn
Donnie Wahlberg as Roman Sionis
John Malkovich as Victor Fries/Mr. Freeze
Scarlett Johansson as Pamela Isley/Poison Ivy
Mickey Rourke as Bane
Gary Oldman as Ras Al Ghul
Derek Mears as Killer Croc
John C. Reilly as Basil Karlo/Clayface
Bradley Cooper as Deadshot