Drake Bell as Peter Parker/Spider-Man
Emily VanCamp as Gwen Stacy
Anton Yelchin as Harry Osborn/Green Goblin II
Hellen Mirren as Aunt May Parker
Elena Satine as Mary Jane Watson
Blake Lively as Felicia Hardy/Black Cat
Eric Johnson as Flash Thompson
Jason Isaacs as Norman Osborn/Green Goblin
J.K Simmons as James Jonah Jamenson
Zooey Deshanel as Betty Brandt
Bill Nunn as Robbie Robertson
Daniel Gillies as John Jamenson
Dylan Baker as Dr. Curt Connors/Lizard
Stephen Moyer as Michael Morbius
Alfred Molina as Dr. Otto Octavius/Dr. Octopus
Thomas Handen Church as Flint Marko/Sandman
Garrett Heldbun as Eddie Brock/Venom
Zack Ward as Cletus Kassady/Carnage
Richard Jenkins as Ben Parker
NOTABLE CAMEOS:
Clark Gregg as Agent Phil Coulson
Donald Glover as Miles Morales/Spider-Man
Karl Urban as Bruce Banner/The Hulk
Ron Eldard as Matt Murdock/Daredevil
Patrick Stewart as Prof. Charles Xavier
Robert Downey Jr. as Tony Stark/Iron Man
Ray Stevenson as Frank Castle/Punisher
Willa Holland as May Parker Watson/Spider-Girl
Samuel L. Jackson or David Hasselhoff as Nick Fury
Julian McMahon as Dr. Victor von Doom/Dr. Doom
BONUS TRACK:
Tanit Phoenix as Sara Pezzini/Witchblade
Idris Elba as Al Simmons/Spawn