The story begins with a teenager narrating about how things have changed since the Superhuman Registration Act enforced by Tony Stark. It then cuts to vigilantes such as Daredevil on the run, or Captain America driving to a different destination. The teenager continues to talk about how heroes and villains are no different from the public eye anymore. The scene changes to the camera slowly zooming out, what's revealed to be a lense, it pans out more and we see a red/blue figure hanging upside down in mid-day.
He free-falls and swings himself from building to building, doing aerial tricks in the process. He lands on a rooftop:
"My name is Peter Benjamin Parker, and I am the Amazing Spider-Man."
Cuts to intro by Marvel Studios
Peter is running late for class and bumps into his best friend, Mary Jane Watson. He shuffles trying to hide his mask as they exchange greetings. Peter smiles and continues to run as he's late for a lecture in Science.
Peter apologizes and tries to catch up on what he's met so far. The scene cuts to after school and Peter is hustling to leave, he puts on his earbuds and listens to the police scanner placed in his phone.
He walks home to pick up spare web shooter cartridges and gets a flashback talking to his Uncle Ben about his Dad's theory on creating a thick enough nylon that can withstand the body's weight, essentially making it the closest reality to flying. It cuts to the present where Peter now suited up as his alter-ego.
As Spider-Man, he stops a robbery and carjacking by armored thugs, sticking them to a lamp-post with the police questioning who it is. Peter swings through the city skyline and finishes more petty crimes before he heads home.
At home he's greeted by his Aunt May and it's Uncle Ben's birthday, they sit together and talk about him. Then another flashback shows all three of them at the dinner table, laughing and talking about how their day went, Peter getting advice from his Uncle about Flash Thompson and Aunt May discouraging the violence. Uncle Ben shrugs as they continue to eat the birthday cake.
It cuts back to the present where Peter is comforting May, and they both hug each other before Peter goes up to his room.
Peter sends an e-mail to The Daily Bugle regarding his interest in sending photos to get published. Peter texts Harry, his best friend, about the struggles so far with Aunt May trying to keep up with the bills and Harry suggests sending some money to help them out, Peter declines politely and looks outside the window to see MJ get hit by her father.
Peter shuts his laptop off, he leans towards to give Mr. Watson a stern look, he backs off and MJ thanks him. The scene cuts to MJ sitting with him on Peter's roof, MJ comforts him and after a while they both say good night to each other.
Meanwhile across the city, a huge man in a white suit is seemingly upset at his failed schemes, he consults another man, one with a huge forehead, yelling at him for messing up. The big man is revealed to be Wilson Fisk, also known as Kingpin. He tells the shorter man, revealed to be Hammerhead to consult his thugs "properly".
The scene cuts to a warehouse, four men are tied up, blindfolded, and bleeding. Hammerhead gives off a lecture on how he's disappointed, and has one of the three people beside Hammerhead, execute the four men. The three henchmen are revealed to be a group called The Enforcers.
Hammerhead tells the group to get rid of the corpses, and gives them an agenda: to find out who Spider-Man is.