It's no secret that THE AMAZING SPIDER-MAN had almost been connected to the Marvel Cinematic Universe when Disney/Marvel Studios and Sony Pictures agreed to include OsCorp Tower as an Easter egg in THE AVENGERS. Unfortunately, timing constraints forced the studios to scrap the idea. Since then, TASM producers Avi Arad and Matt Tolmach has showed interest in continuing there relationship with Disney/Marvel by incorporating Marvel Easter eggs into TASM sequels, Andrew Garfield has expressed interest in being in an Avengers sequel multiple times, and director Webb wasn't shy to admitting he wanted to acknowledge a larger universe in the Blu-ray/DVD commentary of the film. Marc Webb's latest tweet, in response to Sean O'Connell's tweet that Marc Webb is planning to make a massive Spider-Man universe on film, rather cryptically could foreshadow Spider-Man will debut in Marvel's expanded universe.
Some recent transactions between Disney/Marvel and Sony has made fans' spider senses tingle. Of course Disney bought the merchandise rights to the Spider-Man franchise, and it is widely speculated that part of the deal allows them the use of Spider-Man in some form. A character in Marvel Studios' short film ITEM 47 can be seen wearing an Empire State University t-shirt (ESU's film rights are owned by Columbia Pictures/Sony), and ESU should be familiar to Spidey fans as the college Peter Parker attends. A deleted scene from TASM shows Dr. Curt Connors mentioning ESU, and it is rumored The Amazing Spider-Man 2 may feature the school. Even more tantalizing, Target's recent commercial featuring Iron Man and Spider-Man was, for lack of a better word, odd. OsCorp Tower was in fact going to appear in The Avengers, so there it's very plausible that Spider-Man's presence in the MCU could be solidified in TASM2.